Read DNA Profiles from Variation to Blot
DNA profiling uses inherited sequence-length variation, then separates fragments and detects selected patterns with probes.
DNA Fingerprinting and Repetitive DNA is the central idea in this question pattern. Identify the condition in the stem before applying the method below.
Use inherited variation
VNTRs and other noncoding polymorphisms differ among people and are inherited, making multilocus profiles useful for identity and kinship comparisons.
Detect fragments
Restriction fragments are separated by gel electrophoresis, transferred to a membrane by Southern blotting and hybridised with labelled probes. Satellite DNA is highly repetitive, noncoding DNA often concentrated in heterochromatic regions.
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- ✓VNTR variation is inherited and person-variable.
- ✓Southern blot transfers DNA fragments to membrane.
- ✓Probe hybridisation reveals matching sequences.
A blot is the transfer/detection workflow; restriction digestion and hybridisation are separate steps.
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