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Read the Recombinant-DNA Workflow in Order
The workflow moves from DNA cutting and separation to cloning, selection and scale-up.
NSEB · Biotechnology Principles and Processes & Biotechnology and its Applications · 2 linked questions · 100% coverage
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Recombinant DNA Workflow is the central idea in this question pattern. Identify the condition in the stem before applying the method below.
Study deeper: NCERT Biology · Biotechnology Principles and Processes & Biotechnology and its Applications
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Digest DNA with restriction enzymes, separate fragments by gel electrophoresis, isolate the gene of interest, ligate it into a vector, introduce recombinant DNA into host cells, select recombinants, and culture them in a bioreactor. Cohen’s early contribution included plasmid transfer between bacteria.
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- ✓Cut → separate → isolate → ligate → transfer → select → scale up.
- ✓Cohen developed a plasmid-transfer method.
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Do not place bioreactor culture before recombinant selection.
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