Read DNA Gel Electrophoresis
A focused NSEB read for the dna gel electrophoresis pattern. Learn the linked concept, then apply it in the source questions.
DNA Gel Electrophoresis is the central idea in this question pattern. Identify the condition in the stem before applying the method below.
Gel Electrophoresis
Smaller fragments migrate farther toward anode. DNA is negatively charged and migrates toward the anode. Smaller DNA fragments move faster/farther through agarose than larger fragments.
Therefore size order largest to smallest is d>c>a>b. From anode to cathode, the smallest band is farthest toward the anode: b, a, c, d.
Gel Electrophoresis
Pore size and migration of charged DNA fragments. Agarose is obtained from seaweeds, not sea bacteria (A false). DNA fragments separate mainly by size (B true); ethidium-bromide-stained DNA is visualised under UV rather than ordinary visible light (C false); increasing agarose concentration reduces pore size (D true).
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- ✓Smaller fragments migrate farther toward anode
- ✓Pore size and migration of charged DNA fragments
Read the conditions in the stem carefully and use the stated model, labels, or assumptions before choosing an answer.
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